Abstract
Ethnopharmacological relevance: Peltophorum africanum is widely used in indigenous medicine to treat infections,
wounds, and inflammatory conditions. This study employs a bioassay-guided approach to isolate antibacterial
compounds elucidating their pharmacological mechanisms and supporting their potential as sources of antiinfective
agents.
Aim of the study: The study aimed to investigate P. africanum stem as a potential source of novel compounds for
drug discovery, emphasizing its antibacterial, antibiofilm, and antioxidant properties.
Materials and methods: Chromatographic techniques were used to fractionate and isolate antibacterial compounds.
Structural elucidation was performed using NMR and LC-MS. Antioxidant activity was assessed using the
DPPH radical scavenging assay. Antibacterial activity was determined using the microbroth dilution method
against Pseudomonas aeruginosa and Staphylococcus aureus. Anti-biofilm activity was evaluated using the crystal
violet staining method. The antibacterial mechanism of action of the most active fraction was examined by
assessing changes in INT-dehydrogenase activity and monitoring the leakage of intracellular proteins and DNA.
Results: Betulinic acid-3-3,4-dihydroxybenzoate and 3-octadecanoyl stigmasterol were isolated from the hexane
crude extract. LC-MS identified 13 compounds. The plant samples MIC varied from 0.31 to 1.25 mg/mL. Isolated
compounds had noteworthy activity across all biofilm phases. The fraction exhibited the lowest number of viable
cells when assessing INT-dehydrogenase activity. Additionally, it was more effective in causing the release of
intracellular proteins and DNA, while also displaying superior antioxidant activity.
Conclusion: The ability of antioxidant and antibacterial compounds of the P. africanum stem to inhibit biofilm
formation and eradication of mature biofilms holds promise for the treatment of biofilm-associated nosocomial
infections.
wounds, and inflammatory conditions. This study employs a bioassay-guided approach to isolate antibacterial
compounds elucidating their pharmacological mechanisms and supporting their potential as sources of antiinfective
agents.
Aim of the study: The study aimed to investigate P. africanum stem as a potential source of novel compounds for
drug discovery, emphasizing its antibacterial, antibiofilm, and antioxidant properties.
Materials and methods: Chromatographic techniques were used to fractionate and isolate antibacterial compounds.
Structural elucidation was performed using NMR and LC-MS. Antioxidant activity was assessed using the
DPPH radical scavenging assay. Antibacterial activity was determined using the microbroth dilution method
against Pseudomonas aeruginosa and Staphylococcus aureus. Anti-biofilm activity was evaluated using the crystal
violet staining method. The antibacterial mechanism of action of the most active fraction was examined by
assessing changes in INT-dehydrogenase activity and monitoring the leakage of intracellular proteins and DNA.
Results: Betulinic acid-3-3,4-dihydroxybenzoate and 3-octadecanoyl stigmasterol were isolated from the hexane
crude extract. LC-MS identified 13 compounds. The plant samples MIC varied from 0.31 to 1.25 mg/mL. Isolated
compounds had noteworthy activity across all biofilm phases. The fraction exhibited the lowest number of viable
cells when assessing INT-dehydrogenase activity. Additionally, it was more effective in causing the release of
intracellular proteins and DNA, while also displaying superior antioxidant activity.
Conclusion: The ability of antioxidant and antibacterial compounds of the P. africanum stem to inhibit biofilm
formation and eradication of mature biofilms holds promise for the treatment of biofilm-associated nosocomial
infections.
| Original language | English |
|---|---|
| Article number | 119876 |
| Number of pages | 11 |
| Journal | Journal of Ethnopharmacology |
| Volume | 348 |
| DOIs | |
| Publication status | Published - Apr 23 2025 |