TY - JOUR
T1 - Evaluation of the van Deemter equation in terms of open-ended flow to chromatography
AU - Andersen, Jens E.T.
AU - Mukami, Hawa W.
AU - Maina, Irene W.
N1 - Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/8
Y1 - 2020/8
N2 - Plate theory and adsorption theory are the main tools available for understanding chromatographic experiments. Both theories predict a Gaussian distribution of solute molecules within the tubular system. However, Gaussian concentration distributions are observed predominantly at slow linear flow rates, while asymmetric concentration distributions are observed at the linear flow rates most used in chromatography. Allegedly, this asymmetry originates from an inhomogeneous distribution of grain sizes in the column and column overload. However, it is an experimental fact that the distribution of chemicals within an injected volume of solute changes as a function of time, while the response is measured simultaneously. Accordingly, the obtained signal cannot be compared to the theory before some type of time-based deconvolution of the data has been performed. Adjustments to high-performance liquid chromatography data were thus proposed through empirical equations that describe the relevant time values, peak height, peak area, and parameters of the van Deemter equation. It was proposed that the transfer of solute from the front to the rear part of the pulse during laminar open-ended flow occurs at rate that depends on the linear flow rate, and to a lesser extent, on properties of the response function.
AB - Plate theory and adsorption theory are the main tools available for understanding chromatographic experiments. Both theories predict a Gaussian distribution of solute molecules within the tubular system. However, Gaussian concentration distributions are observed predominantly at slow linear flow rates, while asymmetric concentration distributions are observed at the linear flow rates most used in chromatography. Allegedly, this asymmetry originates from an inhomogeneous distribution of grain sizes in the column and column overload. However, it is an experimental fact that the distribution of chemicals within an injected volume of solute changes as a function of time, while the response is measured simultaneously. Accordingly, the obtained signal cannot be compared to the theory before some type of time-based deconvolution of the data has been performed. Adjustments to high-performance liquid chromatography data were thus proposed through empirical equations that describe the relevant time values, peak height, peak area, and parameters of the van Deemter equation. It was proposed that the transfer of solute from the front to the rear part of the pulse during laminar open-ended flow occurs at rate that depends on the linear flow rate, and to a lesser extent, on properties of the response function.
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U2 - 10.1002/jssc.202000413
DO - 10.1002/jssc.202000413
M3 - Article
C2 - 32548959
AN - SCOPUS:85087572245
SN - 1615-9306
VL - 43
SP - 3251
EP - 3265
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 16
ER -