Modeling uncertainty by the principle of pooled calibrations and issues with method validations may influence consensus in science

A comprehensible method validation is required for analytical chemistry and across the disciplines, in order to produce reliable levels of uncertainty of measurements to perform modeling, testing and comparison. New guidelines of quality assurance serve as an aid to determine the uncertainty of measurement with focus on precision rather than focusing on trueness. A method validation of high-resolution continuous source flame-atomic spectrometry (HR-CS FAAS) for analysis of iron and copper using the principle of pooled calibrations (PoPC), that is not part of contemporary guidelines of quality assurance (QA), was used to deliver consensus values to fulfil the requirements to scientific methodology. Improved equations of the PoPC were developed for both the homoscedastic scenario and the heteroscedastic scenario. The analysis of both iron and copper could be allocated to the homoscedastic scenario with unexpected high levels of uncertainties that might be related to both human error and sizeable detector instability.